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KMID : 0385219910010020125
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Korean Journal of Gerontology
1991 Volume.1 No. 2 p.125 ~ p.130
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Effect of Dietary Selenium on Antioxidative Detoxification Mechanism in Liver of Lead Poisoned Rats
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Abstract
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The cellular peroxidative damage due to lead intake and the antioxidative detoxification effect of dietary selenium on it were investigated in liver of rats fed different levels of dietary lead. Levels of dietary lead in the acetate form were 0(control), 200, 1,000, 2,000 and 5,000 ppm. Except control group, four-level of lead diet groups were again subdivided into two, depending on with (200 PS, 1000 PS, 2000 PS, 5000 PS) and without (200 P, 1000 P, 2000 P, 5000 P) 0.5 ppm selenium supplementation. Male rats of Sprague-Dawley strain weighing 141±5g were randomly into total of nine diet groups and fed ad libidum for 4 weeks. Food efficiency and weight gain were lower only in 2,000 ppm and 5,000 ppm lead groups than control group. Superoxide dismutase (SOD) activities in liver were significantly increased in all dietary lead groups, but not different in selenium supplemented groups, compared with control group. Glutathione peroxidase (GPX) activities in liver were significantly decreased by increasing dietary lead levels, but increased by th supplementation of selenium, compared with control. GPX activity ratios between groups with and without selenium, ie, 200 PS and 200 P, 1000 PS and 1000 P, 2000 PS and 2000 P, and 5000 PS and 5000 P groups were 166, 159, 244 and 296% respectively. Reduced glutathione (GSH) contents in liver were decreased and oxidized form (GSSG) increased in dietary lead groups but not different in selenium supplemented ones except 5000 PS, compared with control groups. Liver lipid peroxide values in 200 P, 1000 P, 2000 P and 5000 P groups were higher 87, 111, 164, 339% of that of control, but selenium supplementation reduced these elevated levels by 33, 38, 50, 65%, respectively.
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KEYWORD
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lead, peroxidative damage, selenium, antioxidative detoxification mechanism
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